Title

Development of a Novel GC/MS Method for the Detection of Nicotinamide and Activity of ADP-Ribosylating Toxins

Author

Lindsay R. Lermo, Concordia University - Portland
Thomas O. Munson, Concordia University - Portland
Michael H. Godsey, Concordia University - PortlandFollow

Date of Award

1-1-2011

Document Type

Poster

College

College of Arts & Sciences

Department

Math & Science

Abstract

ADP-ribosylating toxin enzymes break NADH (Figure1) and transfer the ADP-ribosyl group to a residue on a target protein, permanently inactivating or denaturing the protein. This activity is typically detected with a radioassay, which is expensive and requires radioactive materials.

ADP-ribosylation corresponds with the release of nicotinamide. It is possible to detect nicotinamide with a Gas Chromatograph/Mass Spectrometer (GC/MS) (Jacobson, Dame, Pyrek & Jacobson, 1995). The purpose of this study is to measure ADP-ribosylation activity using GC/MS by detecting the liberated nicotinamide. By derivatizing the nicotinamide, the detection limit was lowered to 0.5ng/μl. Control measurements of ADP-ribosylation activity by a cholera toxin protein found low levels of nicotinamide contamination.

Recommended Citation

Lermo, L. R., Munson, T. O., & Godsey, M. H. (2011). Development of a Novel GC/MS Method for the Detection of Nicotinamide and Activity of ADP-Ribosylating Toxins (Thesis, Concordia University, St. Paul). Retrieved from https://digitalcommons.csp.edu/cup_commons_undergrad/214