Title

Activation of NF-κB by Double-Stranded RNA (dsRNA) in the Absence of Protein Kinase R and RNase L Demonstrates the Existence of Two Separate dsRNA-Triggered Antiviral Programs

Authors

Mihail S. Iordanov, Oregon Health Sciences UniversityFollow
John Wong, Oregon Health Sciences University
John C. Bell, University of Ottawa
Bruce E. Magun, Oregon Health Sciences University

Document Type

Article

Publication Date

1-1-2001

Abstract

Double-stranded RNA (dsRNA) of viral origin triggers two programs of the innate immunity in virus-infected cells. One is intended to decrease the rate of host cell protein synthesis and thus to prevent viral replication. This program is mediated by protein kinase R (PKR) and by RNase L and contributes, eventually, to the self-elimination of the infected cell via apoptosis. The second program is responsible for the production of antiviral (type I) interferons and other alarmone cytokines and serves the purpose of preparing naive cells for the viral invasion. This second program requires the survival of the infected cell and depends on the expression of antiapoptotic genes through the activation of the NF-κB transcription factor. The second program therefore relies on ongoing transcription and translation. It has been proposed that PKR plays an essential role in the activation of NF-κB by dsRNA. Here we present evidence that the dsRNA-induced NF-κB activity and the expression of beta interferon and inflammatory cytokines do not require either PKR or RNase L. Our results indicate, therefore, that the two dsRNA-activated programs are separate and can function independently of each other.

Comments

Publication Information.

Iordanov, M. S., Wong, J., Bell, J. C., & Magun, B. E. (2001). Activation of NF-κB by double-stranded RNA (dsRNA) in the absence of protein kinase R and RNase L demonstrates the existence of two separate dsRNA-triggered antiviral programs. Molecular and Cellular Biology, 21(1), 61-72. doi:10.1128/MCB.21.1.61-72.2001

Published In

Molecular and Cellular Biology

Recommended Citation

Iordanov, Mihail S.; Wong, John; Bell, John C.; and Magun, Bruce E., "Activation of NF-κB by Double-Stranded RNA (dsRNA) in the Absence of Protein Kinase R and RNase L Demonstrates the Existence of Two Separate dsRNA-Triggered Antiviral Programs" (2001). CUP Faculty Research. 106.
https://digitalcommons.csp.edu/cup_commons_faculty/106

Source

CU Commons -- Math and Science Department Faculty Research